#Daun miana serial

The callus was maintained for eight weeks. The explant of black betel’s leaf was cultured on Murashige and Skoog medium with four combination treatments (I1.0K1.0 I1.0K1.5 I1B1.5 I0.5B0.5).

This study was aims to determine the effect of variations in growth regulator Indole Acetic Acid (IAA), Benzyl Amino Purine (BAP), and Kinetin on secondary metabolite compounds contained in black betel callus.

Var Nigra (black betel) is one type of medicinal plant that has the potential to be developed by tissue culture method. Berdasarkan analisis GCMS menunjukkan bahwa senyawa metabolit sekunder dengan presentase tertinggi terdapat pada masing- masing perlakuan yaitu senyawa gamma sitosterol (14,88%) pada perlakuan I1,0K1,0 senyawa 14-Beta H Pregna (15,94%) pada perlakuan I1,0K1,5 serta senyawa beta-d-glucopyranoside (15,54%) pada perlakuan I0,5B0,5 dan senyawa beta-d-glucopyranoside (5,63%) pada perlakuan I1,0 B1,5. Simplisia kalus sirih hitam di maserasi menggunakan pelarut metanol dan diidentifikasi menggunakan Gas Chromatography Mass Spectra (GCMS). Eksplan daun dari sirih hitam ditanam pada medium Murashige dan Skoog dengan 4 perlakuan kombinasi (I1,0K1,0 I1,0K1,5 I1B1,5 I0,5B0,5). Penelitian ini bertujuan untuk mengetahui pengaruh variasi zat pengatur tumbuh Indole Acetic Acid (IAA), Benzyl Amino Purine (BAP), Kinetin terhadap senyawa metabolit sekunder yang terdapat kalus sirih hitam. var Nigra (sirih hitam) merupakan salah satu jenis tanaman obat yang berpotensi untuk dikembangkan dengan metode kultur jaringan. It is interesting for further investigate the differences of respon between in vitro and in vivo to determine involved factors.Piper betle L. Unfortunately, the result of invivo assay showed that the chloroform extract treatment with dose level 25 mg/kg BW could not to reduce the number of tapeworm in chicken. The promising activity displayed by a number of extracts has led to further investigation of the active compound. In general, chloroform extract proved to be a more efficient extractant of biologically active compounds than either hexane, ethanol or water extract.

Three of four extracts displayed strong anthelmintic activity with the higest activity belong to chloroform extract with EC50 5 mg/ml followed by n-hexane 9 mg/ml and metanol extract 10,2 mg/ml, while water extract has a weak anthelmintic activity with 106,2 mg/ml. The result of phytochemistry analysis showed that Coleus leaves consisted of flavonoid, steroid, tannin and saponin.

#Daun miana serial

Anthelmintic activity was evaluated with an assay using chicken tapeworm in a serial microplate dilution method by determination of efective concentration 50 (EC50) using probit analysis. Phytochemical analysis was carried out to determine the chemical compound of secondary metabolites. Leave of painted nettle were collected and extracted with hexane, chloroform, ethanol and water. Study on the chemical compound of painted nettle (Coleus blumei Benth) leave extract and its anthelmintic activity against chicken tapeworm were conducted.